mouse pancreatic beta cell line min6  (AddexBio Inc)

Journal: Cell metabolism

Article Title: Expansion of Islet-Resident Macrophages Leads to Inflammation Affecting β Cell Proliferation and Function in Obesity

doi: 10.1016/j.cmet.2018.12.003

Figure Lengend Snippet: (A) Basal insulin secretion and GSIS inMin6 cells after direct cell-cell co-culture with or without intra-islet (CD11c+) or peri-islet (CD11c−) macrophages isolated from age-matched NCD and HFD mice. The cells were co-cultured ata5 (Min6 cells) to 1 (macrophages) ratio to reflect the cell ratio in HFD mouse islets. After 24 hr, insulin secretion was measured as described in the STAR Methods section. n = 4 per group.

Article Snippet: Min6 cell line , ATCC , CRL-11506.

Techniques: Co-Culture Assay, Isolation, Cell Culture

Journal: Cell metabolism

Article Title: Expansion of Islet-Resident Macrophages Leads to Inflammation Affecting β Cell Proliferation and Function in Obesity

doi: 10.1016/j.cmet.2018.12.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Min6 cell line , ATCC , CRL-11506.

Techniques: Recombinant, Isolation, Staining, Red Blood Cell Lysis, Reverse Transcription, SYBR Green Assay, Control, Enzyme-linked Immunosorbent Assay, Software